Figure 4

Micro-CT images of a primo node (PN) obtained from PVS tissue of an anemic rat. (A) CT images of the PN in an anemic rat. (a1) Surface images in grayscale and cross-sectional images in color scale at low and higher magnifications (a2 and a3). Note that the bundles were not well developed in the PN from the anemic rat. (B) The intensity plot of the color scale images on the long (b1) and short axes (b2) of the cross section at the levels marked by green arrows shown in A (a2). (C) Distance of the low-intensity area measured at the level of 30 a.u. (n = 35 and n = 35 from 7 sections in both the control and anemic rats). ***p < 0.001. The control data were obtained from the tissue presented in Figs. 1–3. (D) Short- and long-axis diameters of sinuses in the PN and primo vessel measured at 0 a.u. (n = 16 and 32 measurements from 4 sections). CT = computed tomography; PVS = primo vascular system.