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From: Production and Characterization of Monoclonal Antibodies Against Primo Vascular System of Rat

Immunofluorescence microscopic (IF) images of PV inside lymphatic vessel (LV). Cross (A–C) and longitudinal section (D–F) of LV were probed with α-rPVS-m3-2, and then processed to visualize reaction of immunogen-mAb by 2nd Ab-Dylight 488 (B, E). The signal appeared only on PV but not on LV, indicating that α-rPVS-mAb has specificity to PV of rat in IF. Scale bars indicate 100 µm.