5
From: Production and Characterization of Monoclonal Antibodies Against Primo Vascular System of Rat
Immunofluorescence microscopic (IF) images of PV and PN probed with α-PVS-m3-4. Cross (A–C) and longitudinal section (D–F) of PV and PN (G–H) were probed with α-rPVS-m3-4 and localization of immunogen was detected by 2nd Ab-Dylight 488 (B, E, H). α-rPVS-m3-4 was bound to on cell membrane and extra-cellular matrix of PV and PN (arrow in B, E, H). It was also bound to surface of sub-ducts of PV and PN (arrowhead in B, H). Scale bars indicate 100 µm.