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From: Production and Characterization of Monoclonal Antibodies Against Primo Vascular System of Rat

Immunofluorescence microscopic (IF) images of PV and PN probed with α-PVS-m3-2. Cross (A–C) and longitudinal section (D–F) of PV and PN (G–H) were probed with α-rPVS-m3-2 and localization of immunogen was detected by 2nd Ab (goat anti-mouse IgG)-Dylight 488 (B, E, H). α-rPVS-m3-2 was predominantly localized on cell membrane and extra-cellular matrix of PV (arrow in B, E) and PN (arrow in H). Note that there were two different types of sinus in PN; insets by open and white triangle. Sinus by open triangle was not stained but sinus by white triangle was stained at its outer surface. Scale bars indicate 100 mm.