Figure 3

The percentage of hemolysis in erythrocyte cells induced by H₂O₂ in the presence or absence of different almond hull extracts. (A) The red blood cells (RBCs) were preincubated at 37°C for 45 minutes with different phenolic extracts of almond hull (200 µg/mL) or ascorbic acid (ASC; 30 µg/mL), then the H₂O₂ was added and the percentage of hemolysis was evaluated in a time dependent manner, using a UV/Visible spectrophotometer). (B) The RBCs were treated with acetone extract of almond hull (200 µg/mL) at 37°C for 45 minutes. Cells were washed three times with phosphate buffered saline solution (PBS) and then H₂O₂ was added. After 5 hours incubation, the percentage of hemolysis was evaluated. * Indicates p < 0.05 compared with cells treated solely with H₂O₂. ^† Not significant difference between incubation times.