Figure 4
From: Protocol for Detecting the Primo Vascular System in the Lymph Ducts of Mice
A harvested primo vessel (PV). (A) The stereomicroscopic image of a PV in a lymph duct after extracting it and fixing it with neutral buffered formalin on a slide. The branching of the lymph and primo vessels are clearly visible. The right inset is the stereomicroscopic image of the PV before extraction. (B) Phase contrast microscope image of the PV of the dotted box in (A) at higher magnification. The thickness of the PV is approximately 20 µm. (C) The 4′,6-diamidino-2-phenylindole (DAPI)-stained rod-shaped nuclei (arrows) are aligned along the PV. It is inside a lymph vessel.