Effect of Egg White Combined with Chalcanthite on Lipopolysaccharide induced Inflammatory Cytokine Expression in RAW 264.7 cells

(4) Journal of Pharmacopuncture, Vol.14, No.1, pp.7∼11, 2012

Effect of Egg White Combined with Chalcanthite on Lipopolysaccharide induced Inflammatory Cytokine Expression in RAW 264.7 cells

Eun-A. Choi, Jeung-Won Yoon, Hak-Joo Choi, Dong-Hee Kim, Hwa-Seung Yoo

Abstract

Aim: Historically, mineral compound herbal medicines have long been used in treatments of immune-related diseases in Korea, China and other Asian countries. In this study, we investigated the anti-inflammatory effect of egg white combined with chalcanthite (IS4) on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells.

Methods: RAW 264.7 cells cultured with LPS and various concentrations of IS4 were analyzed to determine the production of pro-inflammatory cytokines and mediators by using enzyme-linked immune sorbent assays (ELISAs).

Results: IS4 concentration inhibited the production of interleukin-1beta (IL-1β), interleukin-6 (IL-6), and granulocytemacrophage colony-stimulating factor (GM-CSF) induced by LPS. IS4 at high concentrations (25 and 50‘µg/ml) inhibited, in concentration-dependent manner, the expression of tumor necrosis factor-alpha (TNF-‘α) stimulated by LPS.

Conclusion: IS4 has shown an anti-inflammatory effect in RAW 264.7 cells.

Key Words: cytokine; GM-CSF; IL-1β; IL-6; lipopolysaccharide; TNF-α